Silvia D’Ambrosi – ESR 1


Main Host Institution: Amsterdam UMC

Host Lab: Neurosurgery Lab

Project Title: Comprehensive characterization of nucleic acid compo-sition in TEPs from NSCLC patients

Project Background: Blood is a rich source of biomarkers which can be used to diagnoses, monitor tumor progression and response to therapy in a minimal-invasive way. Tumor Educated Platelets (TEPs) have shown to be an efficiency biomarker for detect and classify several types of cancers. During my PhD I aim to exploit the potential of TEPs in order develop a liquid biopsy assay for the early detection of NSCLC.

Project Aim:

  • Optimize samples processing and compare RNA isolation methods for optimal total RNA recovery from TEPs in order to maximize sample data output.
  • Optimize RNAseq-based detection of all RNA types for comprehensive analysis in order to maximize biomarker discovery
  • Identify RNA molecules as potential biomarkers and select optimal detection methodologies for biomarker validation and computational analysis for biomarker validation and computational analysis